Scientists at the Max Planck Florida Institute for Neuroscience (MPFI), in collaboration with ZEISS and MetaCell, have introduced Neuroplex, a groundbreaking imaging pipeline that enables the simultaneous monitoring of nine distinct neuronal populations in freely moving mice. This development addresses a critical limitation faced by neuroscientists, as traditional miniscopes could only differentiate two types of neurons at a time. By combining the capabilities of a miniscope and a confocal microscope, researchers can label neurons with various fluorescent tags and capture their activity in real-time. After initial imaging, the miniscope is removed, and a confocal microscope identifies the specific neuron types based on the color tags. This method allows for direct comparison of neural activity patterns across different circuits during behaviors such as social interactions. The researchers reported that 75% of active neurons were accurately classified into specific types, achieving 90% accuracy in their automated classification system. The Neuroplex pipeline not only enhances data collection efficiency but also paves the way for longitudinal studies on learning and disease progression, with plans to make the technique widely accessible to the neuroscience community.